Xiuzhu Sun

　　 　　

　　1. Basic Information

　　Sun xiuzhu (1976-), male, born in wangkui county, heilongjiang province, associate professor, doctoral supervisor. Member of a council animal reproduction branch of Chinese society of animal husbandry and veterinary science.

　　2. Major Research Direction

　　1. Animal genetic breeding and reproduction;

　　2. Reproductive immunology;

　　3. Livestock and grass coupling.

　　3. Courses

　　Undergraduate: Animal reproduction, animal reproduction practice

　　Postgraduate: Animal reproduction technology, Animal embryo engineering technology, Special topic of animal biotechnology, Special topic of animal reproductive physiological regulation, Scientific paper writing for doctor and master

　　4. Education experience

　　1. sep.2004-Jul.2007, China agricultural university, biochemistry and molecular biology, doctor, supervisor: li ning.

　　2. sep.2001-Jul.2004, Northwest university of agriculture and forestry science and technology, animal genetic breeding and reproduction, master, supervisor: li qingwang/zhu huabin.

　　3. Sep.1996-Jul. 2004, bachelor of animal science, northwest university of agriculture and forestry, tutor: li qingwang.

　　5. Research and academic work experience

　　5.1 Work experience

　　1. 2018-present. Associate professor, College of grassland agriculture, northwest university of agriculture and forestry,

　　2. 2012-2018. Associate professor, College of animal science and technology, northwest university of agriculture and forestry,

　　3. 2008-2012. Post-doctoral fellow, University of IOWA, USA, co-supervisor: John Butler.

　　5.2 Lead or participate in scientific research projects

　　1. Shaanxi province key research and development program (2018ZDXM-NY-040)

　　2. Sub-project of national science and technology major project (2018ZX0801106B-003)

　　3. Collaborative Innovation Major Projects of Research and Production Practical Application for Yangling Demonstration Zone (2016CXY-15)

　　5.3 University student science and technology innovation project

　　1. National university student innovation program Identification of indels in PPP2R2B gene in four cattle breeds and thier association with growth traits.

　　2. National university student innovation program Identification of indels in FRZB gene in four sheep breeds and thier association with growth traits.

　　5.4 Representative research results and academic awards

　　Journal article：

　　First author article：

　　(1) Jing-Hua Hu#, Xiu-Zhu Sun#, Qing-Wang Li*, Ting Zhang, Xiao-Chen Hu, Jian-Hong Hu, Li-Qiang Wang, The effect of Laminaria japonic polysaccharide on sperm characteristics and biochemical parameters in cryopreserved boar sperm. Animal Reproduction Science , 2013, 139(1-4): 95-100.

　　(2) Hualin Cao#, Xiuzhu Sun#, Qingwang Li*, Shengxian Zhou, Xiangbin Nan, Jianhong Hu, Liqiang Wang, Yamei He, Testing Rhodiola sachalinensis saccharide as cryoprotectant for bovine spermatozoa,  Journal of Dairy Science ,  2013, 96(11): 6965-6972.

　　(3) Xiu-Zhu Sun, J.E. Butler* and Nancy Wertz, Antibody Repertoire Development in Fetal and Neonatal Piglets XXIII: Fetal piglets infected with a vaccine strain of PRRS Virus display the same immune dysregulation seen in isolator. Vaccine , 2012, 30(24): 3646-3652.

　　(4) X-Z Sun, N. Wertz, M. Sinkora, K. Lager, G. Tobin and J.E. Butler*. Antibody repertoire development in fetal and neonatal piglets. XXII. λ Rearrangement precedes κ rearrangement during B-cell lymphogenesis in swine, Immunology , 2012, 137(2):149-59.

　　(5) X-Z Sun, N. Wertz, K. Lager and J.E. Butler*, Antibody repertoire development in fetal and neonatal piglets. XV: Immunoglobulin levels and antibody responses in isolator piglets infected with porcine circovirus type 2,  Vaccine , 2012, 31(1): 141-148.

　　(6) Sun X, Wang S, Zhang Y, Wang H, Wang L, Ying L, Li R, Li N*, Cell-cycle synchronization of ibroblasts derived from transgenic cloned cattle ear skin: effects of serum starvation, roscovitine and contact inhibition. Zygote . 2008, 16(2): 111-116.

　　(7) SunXiuZhu, LiQingWang, DaiYunPing, ChengJinHua, GongGuoChun, WanRong , Zhu HuaBin, Isolation and Culture in Lu-Xi cattle-ear skin fibroblast and epithelial cells,  Journal of Veterinala and Zoote chnica. 31, (26) 2004.

　　(8) Sun XZ, Zhu YJ, Du LJ, Wang LQ, Kang MY, Wang SH. Advances in the isolation and culture of spermatogonial stem cells in vitro. Journal of China agricultural university. 2017, 22(04): 91-100. (Chinese)

　　(9) Jiang XJ#, Sun XZ#, Zhu HB, Du WH, Hao HS, Zhao HS, Zhao XM, Wang D, Liu Y. Development of radioimmunoassay kit for green fluorescent protein. Chinese medical biotechnology. 2016,11(03):272-274.

　　Corresponding author articl：

　　(1) Du LJ, Wang SH, Zhu YJ, Zhao HD, Basit A, Yu XH, Li QW and Sun XZ*. 2018. Immunoglobulin Heavy Chain Variable Region Analysis in Dairy Goats Immunobiology. 2018, 223(11): 599-607.

　　(2) Zhao HD＃, He S＃, Wang SH, Zhu YJ, Xu HW, Luo RY, Lan XY, Cai Y, Sun XZ*. 2018. Two New Insertion/Deletion Variants of the PITX2 Gene and their Effects on Growth Traits in Sheep. Anim Biotechnol. 2018, 29(4): 276-282.

　　(3) Zhao HD, Wu ML, Wang SH, Yu XH, Li Z, Dang RH*, Sun XZ*. 2018. Identification of a novel 24-bp insertion/deletion (InDel) of the androgen receptor gene and its association with growth traits in four indigenous cattle breeds.  Arch. Anim. Breed . 61:71~78.

　　(4) Zhao HD, He S, Zhu YJ, Cao X, Luo RY, Cai Y, Xu HW*, Sun XZ*. 2017. A novel 29?bp insertion/deletion (InDel) variant of the LHX3 gene and its influence on growth traits in four sheep breeds of various fecundity. Arch. Anim. Breed . 60, 79~85.

　　(5) Huang LH, Li XR, Long C, Shi NN, Feng C, Du MJ, Zhu HB, Pan DK, Sun XZ*. CRISPR/Cas9 Mediated CMAH Gene Knockout and Breeding Analysis of Pigs (Sus scrofa).  Journal of Agricultural Biotechnology , 2018, 26(06): 1064-1073.

　　(6) He S, Wang SH, Sun XZ*. Probe into Application of CRISPR/Cas9 Technology in Epigenome. Biotechnology Bulletin , 2018, 34(05): 17-21.

　　(7) Du LJ, Wang SH, Zhu YJ, Kang MY, Li QW, Sun XZ*. Research Progress of Livestock Immunoglobulin Gene Expression Repertoire. Journal of Domestic Animal Ecology, 2016, 37(05):78-84.

　　(8) Du LJ, Wang SH, Zhu YJ, Kang MY, Zhao HD, Zheng K, Sun XZ*. Somatic Hypermutation Diversity in the Goat Immunoglobulin Heavy Chain Variable Region Repertoire. Journal of Domestic Animal Ecology , 2016, 37(11): 17-24+96.

　　Other article:

　　(1) J.E. Butler*, X-Z Sun, N. Wertz, K.M. Lager, Antibody repertoire development in fetal and neonatal piglets. XVI. Influenza stimulates adaptive immunity, class switch and diversification of the IgG repertoire encoded by downstream Cγ genes, Immunology, 2013, 138(2): 134-144.

　　(2) J.E. Butler*, X-Z Sun, N. Wertz , K.M. Lager, K, Chaloner and G. Tobin, Antibody repertoire development in fetal and neonatal piglets XXI: Usage of most VH genes remains constant during fetal and postnatal development, Molecular Immunology, 2011, 49(3): 483-494.

　　(3) John E. Butler*, Kristina Santiago-Mateo, Xiu-Zhu Sun, Nancy Wertz,  Marek Sinkora and David H. Francis, Antibody Repertoire Development in Fetal and Neonatal Piglets. XX. B Cell Lymphogenesis Is bsent in the Ileal Peyer’s Patches, Their Repertoire Development Is Antigen Dependent, and They Are Not Required for B Cell Maintenance, The Journal of Immunology, 2011, 187(10): 5141-5149.

　　(4) M. Mendicino, J. Ramsoondar, C. Phelps, T. Vaught, S. Ball, T. LeRoith, J. Monahan, S. Chen, A. Dandro, J. Boone, P. Jobst, A. Vance, N. Wertz, Z. Bergman, X-Z. Sun, I. Polejaeva, J. Butler, Y. Dai, D. Ayares, K. Wells, Generation of antibody and B cell-deficient pigs by targeted disruption of the J-region gene segment of the heavy chain locus, Transgenic Res, 2011, 20(3): 625-641.

　　(5) Tomoko Eguchi-Ogawa, Nancy Wertz, Xiu-Zhu Sun, Francois Puimi, Hirohide Uenishi, Kevin Wells,Patrick Chardon, Gregory J. Tobin, and John E. Butler*, Antibody Repertoire Development in Fetal and Neonatal Piglets. XI. The Relationship of Variable Heavy Chain Gene Usage and the Genomic Organization of the Variable Heavy Chain Locus, The Journal of Immunology, 2010, 184(7): 3734-3742.

　　(6) S. Wang, X. Sun, F. Ding, K. Zhang, R. Zhao, S. Li, R. Li, B. Tang,L. Zhang, Y. Liu, J. Li, F. Gao, H. Wang, L. Wang, Y. Dai , N. Li*, Removal of selectable marker gene from fibroblast cells in transgenic cloned cattle by transient expression of Cre recombinase and subsequent effects on recloned embryo development. Theriogenology, 2009, 72 (4): 535-541.

　　(7) He Z, Sun X, Mei G, Yu S, Li N*, Nonclassical secretion of human catalase on the surface of CHO cells is more efficient than classical secretion, Cell Biol Int, 2008, 32(4): 367-373.

　　(8) Yang P, Wang J, Gong G, Sun X, Zhang R, Du Z, Liu Y, Li R, Ding F, Tang B, Dai Y, Li N, Cattle mammary bioreactor generated by a novel procedure of transgenic cloning for large-scale production of functional human lactoferrin PLoS One, 2008, 3(10): e3453.

　　(9) Liu Y, Wu Q, Cui H, Li Q, Zhao Y, Luo J, Liu Q, Sun X, Tang B, Zhang L, Dai Y, Li N. Expression of EGFP and NPTII protein is not associated with organ abnormalities in deceased transgenic cloned cattle, Cloning Stem Cells, 2008, 10(4): 421-428.

　　(10) He Zu-Yong, Sun Xiu-Zhu, Zhao Yong-Hui, Li Ning*, Preparation of microcells for use in production of transchromosomic animal, Progress in biochemistry and biophysics, 2007, 34(7): 770-776.

　　(11) Kun Z, Shaohua W, Yufang M, Yankun L, Hengxi W, Xiuzhu S, Yonghui Z, Yan L, Yunping D, Lei Z, Ning L, Effects of leptin supplementation in in vitro maturation medium on meiotic maturation of oocytes and preimplantation development of parthenogenetic and cloned embryos in pigs, Anim Reprod Sci, 2007, 101(1-2): 85-96.

　　(12) Zhang YH*, Pan DK*, Sun XZ, Sun GJ, Wang XB, Liu XH, Li Y, Dai YP, Li N*, Production of porcine cloned transgenic embryos expressing green fluorescent protein by somatic cell nuclear transfer, Sci China C Life Sci, 2006, 49(2): 164-171.

　　(13) Zhang Yun-Hai*, Pan Deng-Ke*, Sun Xiu-Zhu, Sun Guo-Jie, Liu Xiao-Hui, Wang Xiao-Bo, Tian Xing-Hua, Li Yan, Dai Yun-Ping, Li Ning, In Vitro Developmental Competence of Pig Nuclear Transferred Embryos: Effects of GFP-transfection, Refrigeration, Cell Cycle Synchronization, and Shapes of Donor Cells, Zygote, 2006, 14(3): 239-247.

　　(14) Pan D*, Zhang Y*, Sun XZ, Zhang J, Li X, Li Y, Gu Z, Dai Y, Wu C, Li N, Cloned pigs derived from somatic cell nuclear transfer embryos cultured in vitro at low oxygen tension. Chinese Science Bulletin, 2006, 51(7): 839-844.

　　(15) Pan Deng-Ke, Zhang Yun-Hai, Sun Xiu-Zhu, Li Yan, Li Yan ,Li Ning*, Effects of Donor Cells on in vitro Development of Porcine Cloned Embryos. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2006, 37(4): 331-336.

　　(16) He ZY, Sun XZ, Li N. Production of transchromosomes using MMCT technology. China Bioengineering Society, Jilin Agricultural University. China Bioengineering Society 2006 Academic Annual Meeting and National Bioreactor Symposium Abstract Collection. China Bioengineering Society, Jilin Agricultural University:,2006:1.

　　(17) Zhao HD, Wu ML, Sun XZ, Wang SH*. Polymorphism Analysis of 3'-UTR Sequence of Chinese Yellow Cattle PSAP Gene. 2017.Proceedings of the 19th National Symposium on Animal Genetics and Breeding. 564.

　　(18) Zhao HD, Wu ML, Sun XZ, Wang SH*. Effects of tissue source and culture methods on the establishment of bovine adipocyte culture system. 2017. Proceedings of the Tenth National Animal Biotechnology Symposium. 221.

　　(19) Wu ML, Zhao HD, Wang SH, Sun XZ*. Identification of LYST gene indel in yellow cattle and its genetic effects. The 19th Symposium of the Animal Breeding Branch of the Chinese Society of Animal Husbandry and Veterinary Medicine. 283

　　(20) Ze Li, Luejie Fan, Shuhui wang, Xiuzhu Zhu*. Association ananlysisi of PLAG1 gene and indel with growth traits in chinese cattle. The 19th Symposium of the Animal Breeding Branch of the Chinese Society of Animal Husbandry and Veterinary Medicine. 323

　　(21) Haidong Zhao, Mingli Wu , Xiuzhu Sun, Shuhui Wang* . Identification of novel isoforms of bovine PSAP gene and their mRNA expression characterization to  lipid metabolism.The 19th Symposium of the Animal Breeding Branch of the Chinese Society of Animal Husbandry and Veterinary Medicine.315

　　Book:

　　Sun XZ (Participation) J. E. Butler*, Xiu-Zhu Sun and Nancy Wertz. GENE DUPLICATION: Immunoglobulin Polygeny--An Evolutionary Perspective. Published by InTech. ISBN 978-953-307-387-3; 2011.

　　Sun XZ (Associate editor)  Key technologies for modern deer and deer product processing, Zhongyuan Farmers Publishing House, 2016.

　　Sun XZ (Participation) Dairy farm production management technology, China Agricultural University Press, 2003.

　　Sun XZ (Participation) Animal reproductive immunology, Northwest Agriculture and Forestry University Press 2011.

　　Sun XZ (Participation) Cell Engineering Principles and Techniques, Higher education press, 2017.

　　Sun XZ (Participation) China Grassland Productivity and Food Safety Research, Science Press, 2017.

　　Academic reward：

　　Cow mammary gland bioreactor for efficient expression of recombinant human lactoferrin and a-whey albumin.Ministry of Education Science and Technology Second Prize, 10th Completion, Certificate No: 2005-192.

　　Research and application of somatic cell cloning of top Holstein bulls.The certificate of completion of scientific and technological achievements of the Ministry of Education, the 12th completion person, the result registration number: 360-04-20080517-12.

　　Dairy cow mammary gland bioreactor for efficiently expressing medicinal health proteins such as human lactoferrin.Certificate of Completion of Scientific and Technological Achievements of the Ministry of Education, 10th Completion, Result Registration Number:360-05-20080306-10.

　　China Agricultural Science and Technology Award (Excellent Innovation Team Award (equivalent to the first prize of scientific research))Meat and Milk Cattle Genetic Improvement and Germplasm Innovation Team (14th Completion), Certificate No:TD2015-R-012-14.

　　5.5 Teaching and teaching reform projects and rewards:

　　Teaching reform project

　　The second batch of school-level online course construction projects of Northwest Agriculture and Forestry University  Animal reproduction  SPOC. In research, hosted.

　　Northwest Agricultural and Forestry University Teaching Reform Research Project，Animal Breeding Course in English (Cultivation)，2014/06-2016/06, Completed, hosted.

　　Northwest Agricultural and Forestry University Teaching Reform Research Project，Study on the Application Effect of Virtual Simulation Visualization Operation in Animal Breeding Internship Course, 2015/06-2017/06, Completed, hosted.

　　Northwest Agricultural and Forestry University Teaching Reform Research Project，Application of BOPPPS Teaching in Economic Animal Production，2015/06-2017/06，Completed, participant.

　　National video open class The mystery of animal reproduction Lecturer teacher

　　Teaching reward

　　2017 Northwest A&F University “My Favorite Teacher”

　　2016 College of Animal Science and Technology, Northwest A&F University Teaching Reform Project Award

　　2015 The 10th Young Teachers Lecture Competition of Northwest Agriculture and Forestry University  Third prize

　　2015 Young Teachers' Lecture Competition, College of Animal Science and Technology, Northwest A&F University  First prize

　　2015 Advanced Individuals in Ideological and Political Education, Northwest A&F University

　　2015 Excellent Teaching Plan of College of Animal Science and Technology, Northwest A&F University  second prize

　　2015 Excellent Teacher, College of Animal Science and Technology, Northwest A&F University

　　2014 The first micro-teaching competition of Northwest Agriculture and Forestry University  Third prize

　　7 Contact Information

　　Address：Room number: 248, College of Animal Science and Technology, Northwest A&F University, No.22, Xinong Road, Yangling, Shaanxi

　　Postcode：712100

　　Email: sunxiuzhu208@163.com

　　sunxiuzhu@nwsuaf.edu.cn